{"id":38,"date":"2018-11-05T23:49:56","date_gmt":"2018-11-05T23:49:56","guid":{"rendered":"http:\/\/creativelab.hu\/?page_id=38"},"modified":"2019-06-11T11:01:55","modified_gmt":"2019-06-11T11:01:55","slug":"szolgaltatasok","status":"publish","type":"page","link":"https:\/\/www.creativelab.hu\/hu\/szolgaltatasok\/","title":{"rendered":"Szolg\u00e1ltat\u00e1sok"},"content":{"rendered":"

Szolg\u00e1ltat\u00e1sok<\/strong><\/h2>\n

HPV diagnosztika<\/strong><\/p>\n

K\u00fcl\u00f6nb\u00f6z\u0151 DNS konstrukci\u00f3k el\u0151\u00e1ll\u00edt\u00e1sa<\/strong><\/p>\n

25+ \u00e9vnyi tapasztalattal rendelkez\u00fcnk a k\u00fcl\u00f6nb\u00f6z\u0151 rekombin\u00e1ns technik\u00e1k ter\u00fclet\u00e9n. Egy\u00fctt fejl\u0151dt\u00fcnk \u00e9s saj\u00e1t\u00edtottuk el a k\u00fcl\u00f6nb\u00f6z\u0151 restrikci\u00f3s enzim, valamint PCR alap\u00fa kl\u00f3noz\u00f3 technik\u00e1kat, eg\u00e9szen a mai sz\u00ednvonalat jelent\u0151 technik\u00e1kig (pl. Gibson-assembly). A kl\u00f3noz\u00e1s a minden\u00fcnk! Ha sz\u00fcks\u00e9ge van b\u0151vebb inform\u00e1ci\u00f3ra, k\u00e9rem l\u00e9pjen vel\u00fcnk kapcsolatba az info@creativelab.hu e-mail c\u00edmen.<\/p>\n

We have a 25+ years of experience in recombinant techniques. We witnessed and mastered the classic restriction enzyme based cloning techniques and PCR-based methods, followed by the current waves like Gibson-assembly. We love cloning! If you are interested in more details please contact us at info(at)creativelab.hu address.<\/p>\n

Feh\u00e9rje termel\u00e9s \u00e9s tiszt\u00edt\u00e1s E.coli sejtb\u0151l.<\/b><\/p>\n

E.coli a legelterjedtebb<\/p>\n

E. coli<\/em>\u00a0is the most widely used expression host for heterologous protein expression. It is the best characterized organism and there are ample tools available for manipulation and efficient protein production. High protein yield can be cost-efficiently produced by\u00a0E. coli<\/em>. Scale-up is significantly easier than in eukariotic expression hosts, however post-translational modifications are lacking.<\/p>\n

High expression levels often lead to insoluble protein producion. Studies show that less than 50 % of bacterial proteins and less than 15 % of non-bacterial proteins can expressed in soluble form. However, a number possible techniques and our experience can improve the outcome, and\u00a0in vitro<\/em>\u00a0refolding is often successful to prepare active proteins. If you are interested in more details please contact us at info(at)creativelab.hu address.<\/p>\n

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Protein production and purification from CHO cells<\/strong><\/p>\n

Due to its attractive characteristics, Chinese Hamster Ovary (CHO) cells are\u00a0 biotechnology\u2019s workhorses. First of all, CHO cells are quite easily maintained and can grow to very high densities in suspension cultures and in large bioreactors. They produce correctly folded and post-translationally modified (e.g. glycosilated) proteins that show good compatibility and bioactivity in humans. Transgenes can be introduced and amplified efficiently in the CHO genome reaching high copy numbers of the gene of interest. The more than two decades of research enables the production of proteins with high yields even up to 5 g\/L.<\/p>\n

Despite the availability of numerous expression hosts, CHO cells have become the most important cell system for the biotechnological of pharmaceuticals. About 70% of all recombinant therapeutic proteins are produced in mammalian cell lines, and CHO is the dominant choice for antibody production. We offer a stable cell line production service in DG44 licensed from Dr. Lawrence\u00a0Chasin.<\/em>\u00a0Our developped technology and kow-how enables the formation of\u00a0 high copy clones with decent protein expression levels. If you are interested in more details please contact us at info(at)creativelab.hu address.<\/p>\n

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Szolg\u00e1ltat\u00e1sok HPV diagnosztika K\u00fcl\u00f6nb\u00f6z\u0151 DNS konstrukci\u00f3k el\u0151\u00e1ll\u00edt\u00e1sa 25+ \u00e9vnyi tapasztalattal rendelkez\u00fcnk a k\u00fcl\u00f6nb\u00f6z\u0151 rekombin\u00e1ns technik\u00e1k ter\u00fclet\u00e9n. Egy\u00fctt fejl\u0151dt\u00fcnk \u00e9s saj\u00e1t\u00edtottuk […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"acf":[],"yoast_head":"\nSzolg\u00e1ltat\u00e1sok - Creative Labor Kft.<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.creativelab.hu\/in-vitro-diagnostics\/\" \/>\n<meta property=\"og:locale\" content=\"hu_HU\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Szolg\u00e1ltat\u00e1sok - Creative Labor Kft.\" \/>\n<meta property=\"og:description\" content=\"Szolg\u00e1ltat\u00e1sok HPV diagnosztika K\u00fcl\u00f6nb\u00f6z\u0151 DNS konstrukci\u00f3k el\u0151\u00e1ll\u00edt\u00e1sa 25+ \u00e9vnyi tapasztalattal rendelkez\u00fcnk a k\u00fcl\u00f6nb\u00f6z\u0151 rekombin\u00e1ns technik\u00e1k ter\u00fclet\u00e9n. 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